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From 2013.igem.org

Gel Results from the digestion performed on the 20/09/13

Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);

• The gel shows that digestions were successful as every sample dropped the inserted gene.

Gel Order:

F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).

• Same result as the gel above

Evaporation of isolated plasmids and re-suspended in 1xTE

• The samples were evaporated with a vacuum evaporator.
• The samples were re-suspended in 20 ul of 1xTE.

Sample	Volume (ul)	Concentration (ng/ul)	280/260	260/230
B4	20	12.1	1.49	1.44
B9	20	16.3	1.63	1.45
B12	20	26.3	1.87	1.05
X3	20	27.3	1.78	1.11
X5	20	15.6	1.6	1.35
X6	20	25.2	1.63	1.46
F1	20	38.9	1.83	1.57
F10	20	33.6	1.67	1.4
F12	20	6.5	1.48	1.10

Luria Agar plates

• Luria agar plates were made for the plating of the CSE kits that arrived.