User contributions

From 2013.igem.org

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• 21:50, 4 October 2013 (diff | hist) Team:Paris Saclay/Project ‎ (→Detection and degradation of PCB system in Escherichia coli) (top)
• 22:36, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/26 ‎ (→1 - Digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI/PstI)
• 22:35, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/23 ‎ (→1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI)
• 22:35, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 ‎ (→2 - Electrophoresis of gel purification of the digestion of BBa_J04450 by EcoRI/PstI)
• 22:34, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 ‎ (→1 - Gel purification of the digestion of BBa_J04450 by EcoRI/PstI)
• 22:34, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 ‎ (→1 - Plasmid extraction of BBa_K1155000 from DH5α)
• 22:33, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 ‎ (→Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3)
• 22:33, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 ‎ (→2 - Digestion of BBa_K1155000 by Spe I)
• 22:32, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/20 ‎ (→1 - Electrophoresis of PCR products : BphR2 Part I)
• 22:32, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/19 ‎ (→1 - Electrophoresis gel of PCR products : BphR2 Part I)
• 22:31, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/19 ‎ (→3 - Transformation of NarK, NarG or NirB with RBS-LacZ-Term or RBS-AmilCP-Term in PSB1C3 in DH5α)
• 22:31, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→2 - Electrophoresis of PCR products : BphR2 Part I)
• 22:30, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→8 - Electrophoresis of the digestion of BBa_K1155003, BBa_K1155007 by XBaI/PstI)
• 22:29, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→6 - Electrophoresis to check the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI and EcoRI/SpeI)
• 22:27, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→2 - Extraction of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 from DH5α)
• 22:27, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→3 - Electrophoresis of PCR product : BphR2 Part I)
• 22:26, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→2 - Electrophoresis of PCR products : FRN Part I, FNR Part II, BphR2 Part I)
• 22:25, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→2 - Electrophoresis to check the digestion of BBa_J04450 by EcoRI/PstI)
• 19:42, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→4 - Electrophoresis to check the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI and by SpeI/EcoRI)
• 19:41, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→1 - Electrophoresis to check the digestion of BBa_K1155000 by SpeI/PstI)
• 19:33, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→2 - Electrophoresis to check the digestion of BBa_J04450 by EcoRI/PstI)
• 19:33, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→1 - Digestion of BBa_J04450 by EcoRI/PstI)
• 19:32, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→5 - Liquid culture of MG1655Z1 Δfnr::Km)
• 19:30, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3)
• 19:29, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→4 - Electrophoresis to check the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by Spe I and by Spe I/EcoRI)
• 19:28, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→3 - Digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI and by SpeI/EcoRI)
• 19:28, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→2 - Extraction of BBa_K1155004, BBa_1155005, BBa_K1155006 from DH5αstrain)
• 19:28, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→2 - Extraction of BBa_K1155004, BBa_1155005, BBa_K1155006 from DH5α)
• 19:27, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 ‎ (→1 - Electrophoresis to check the digestion of BBa_K1155000 by SpeI/PstI)
• 19:26, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/12 ‎ (→1 - Electrophoresis of PCR products : RBS-BphR2 Part I, BphR2 Part I, BphR2 Part II, RBS-FNR Part I, FNR Part I and FNR Part II) (top)
• 19:25, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/12 ‎ (→3 - Digestion of BBa_K1155000 by Spe I/Pst I)
• 19:24, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/12 ‎ (→2 - Electrophoresis to check the digestion of BBa_K1155000 by SpeI/PstI, BBa_K1155007 and BBa_K1155003 by XBalI/PstI)
• 19:23, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/12 ‎ (→1 - Digestion of BBa_K1155000 by SpeI/PstI, BBa_K1155007 and BBa_K1155003 by XBaI/PstI)
• 19:21, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/9 ‎ (→1 - Electrophoresis of the PCR of BphR2 Part I, BphR2 Part II, RBS_BphR2 Part I, FNR Part I, FNR Part II, RBS_FNR Part I to check the gel purification)
• 19:20, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/9 ‎ (→1 - Extraction of BBa_K115007 from DH5α)
• 19:19, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/9 ‎ (→1 - Tranduction of Km in MG1655Z1)
• 19:15, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→1 - Extraction of plasmid pSB1C3 containing BphR2 ,plasmid pSB1C3 containingFNR and plasmid pSB1C3 contaning RBS-FNR in competent cell DH5α)
• 19:14, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→1 - Extraction of plasmid of BphR2, FNR, RBS-FNR)
• 19:11, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→4 - Transformation of ligation of Pfnr with RBS-LacZ-Term in PSB1C3 in DH5α)
• 19:10, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→5 - Ligation of Pfnr with RBS-LacZ-Term in PSB3K3 and NarK with RBS-LacZ-Term in PSB3K3)
• 19:10, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→3 - Ligation of Pfnr with RBS-LacZ-Term in pSB1C3)
• 19:10, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→2 - Results of culture of Pfnr with RBS-Amil CP-Term in pSB1C3, NirB with RBS-LacZ-Term in pSB1C3 in aerobic and anaerobic conditions)
• 19:10, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→1 - Results of liquid culture of Pfnr-RBS-Amil CP-Term in PSB1C3 in aerobic and anaerobic conditions)
• 19:09, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→7 - Electrophoresis to check the digestion of BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI/PstI and plasmids already digested by SpeI and after digested by PstI)
• 19:08, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→2 - Electrophoresis to check the colony PCR products : BBa_K1155007)
• 19:08, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→1 - Colony PCR of BBa_K115007 in DH5α)
• 19:03, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→3 - Electrophoresis of BBa_J004450 digested by EcoRI/PstI)
• 19:02, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 ‎ (→2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion)
• 19:02, 3 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/7 ‎ (→1 - Tranduction of Km in MG1655Z1)
• 19:01, 3 October 2013 (diff | hist) Team:Paris Saclay/transduction ‎ (→Protocol : Transduction)

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