Team:Paris Saclay/Notebook/August/23

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Contents

1 Notebook : August 23
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
    - 1.1.1.1 Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3
    - 1.1.1.2 1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI

Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3

1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI

Well 1 : 6µL DNA Ladder
Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye
Gel : 1%

Expect sizes :

pSB3K3 : 2750 bp

We can't see anything. The digestion didn't work.

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