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Team:Paris Saclay/Notebook/August/7
From 2013.igem.org
Notebook : August 7
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003
1 - Sequensis analysis of BBa_K1155003
Abdou
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The sequence analysis was good. We call our biobrick BBa_K1155003. |
Objective : obtaining BBa_K1155007
1 - Ligation of RBS-LacZ-Term in pSB1C3
Anaïs, Nadia
Used quantities :
- RBS-AmilCP : 2µL
- Term in pSB1C3 : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
2 - Tranformation BBa_K115507 in DH5αstrain
XiaoJing
Protocol : Bacterial transformation
Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Tranduction of Km in MG1655Z1
Nadia, XiaoJing
Protocol : Transduction
Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.
We did the first step of the protocol : bacteriophage stock grown on BW1328.
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