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  • Team:TU-Munich/Notebook/Labjournal
    * Miniprep was performed after manufacturer's protocol (QIAprep Miniprep, QIAGEN) Sequencing batch were prepared after manufacturer's protocol. (15 µl of plasmid DNA (50 - 100 ng) and 2 µl sequencing p
    804 KB (118,126 words) - 22:30, 22 October 2013
  • Team:Newcastle/Notebook/calendar
    ...tebook/protocols#Escherichia_coli_Transformation ''E.coli'' Transformation Protocol ]. ...m.org/Team:Newcastle/Notebook/protocols#QIAprep_Spin_Miniprep_Kit miniprep protocol], and checked the concentration of plasmids through the use of [https://201
    113 KB (17,146 words) - 14:46, 30 September 2013
  • Team:Paris Bettencourt/Notebook
    <TR><TD><b>Thermocycler Protocol: NEB Phusion</b></TD><TD> </TD><TD> </TD><TD> </TD><TD> </TD></TR> Thermocycler Protocol : NEB Quick-Load<br>
    522 KB (75,300 words) - 03:51, 29 October 2013
  • Team:SDU-Denmark/Tour41
    ...gem.org/mediawiki/2013/0/0b/SDU2013_Protocol_Template.pdf" target="_blank">Protocol Template</a><br> ...org/mediawiki/2013/8/8a/SDU2013_Protocol_0001.pdf" target="_blank">SDU2013 Protocol 0001 - USER primer design</a><br>
    18 KB (2,653 words) - 23:01, 28 October 2013
  • Team:TU-Eindhoven/LabJournal
    ...f different tasks, each of which is performed according to a very specific protocol where even the smallest adaptations can have drastic effects. ...or the ampicillin plates and a 400mL mixture for the kanamycin plates. The protocol we followed for the creation of the agar solutions was as follows:
    244 KB (39,000 words) - 09:06, 26 October 2013
  • Team:UC-Santa Cruz/Notebook1
    ''' General PCR Protocol''' https://www.neb.com/protocols/2012/09/13/protocol-for-phusion-high-fidelity-pcr-master-mix-with-gc-buffer-m0532
    71 KB (11,188 words) - 04:00, 28 September 2013
  • Team:Freiburg/Project/toolkit
    ...smids, you will have to clone your target sequence into our crRNA plasmid (protocol see below). ...ef="https://2013.igem.org/Team:Freiburg/protocols#Transformation">standard protocol</a>. Pick clones, miniprep the plasmids and sequence it with pSB1C3 reverse
    87 KB (13,257 words) - 00:59, 29 October 2013
  • Team:Leicester/Notebook
    Master Mix (changes were made from the protocol)<br /> Protocol:<br />
    117 KB (15,598 words) - 00:32, 5 October 2013
  • Team:BGU Israel/Experiments
    The Electroporation standard protocol was used with the following data:</br> The vials were put in the PCR for 30 cycles. We used the protocol &rdquo;Routine high-fidelity amplification of longer DNA fragments is chall
    71 KB (8,958 words) - 00:46, 29 October 2013
  • Team:UChicago/Notebook
    :*Ivan & Ethan are in charge of the IBC protocol. ::*We can’t start wet lab work until our IBC protocol is finished!
    68 KB (10,479 words) - 02:05, 28 September 2013
  • Team:Freiburg/Notebook/lab multiple targeting
    1h in 1 aliquot of Gibson mix (according to protocol)<br> → <b> pIG7001a </b>, heat shock transfection in E. coli (according to protocol) </p>
    54 KB (7,347 words) - 05:12, 28 October 2013
  • Team:UNITN-Trento/JS/Protocols
    /*Protocol Spoiler*/ $(".tn-spoiler-protocol div").not(".tn-spoiler").slideUp();
    2 KB (163 words) - 16:38, 8 July 2013
  • Team:British Columbia/Notebook/Flavours
    '''Aim:''' Frances and I used the PCR protocol to extract PAL and COMT form the PSB1C3 vector which was miniprepped by Joe ...d in a 1% agarose gel for the 4CMH and the COMT in accordance with our PCR protocol.
    39 KB (6,228 words) - 03:33, 29 October 2013
  • Team:UChicago/Protocols
    #'''Reference to protocol''': are you using the standard protocol found in the masterlist? ...Changes in protocol''': have you done anything different from the standard protocol?
    20 KB (3,354 words) - 21:58, 25 October 2013
  • Team:NTNU-Trondheim/Notebook/July
    ...ding to our <a href="https://2013.igem.org/Team:NTNU-Trondheim/Protocols"> protocol</a>.<br> ...aration</a> continued with completion of step 2-7 in the vesicle isolation protocol. There where made three cell cultures in step 2; ''E.coli'' ER2566 in LB, '
    45 KB (7,110 words) - 20:56, 4 October 2013
  • Team:MIT/MaterialsAndMethods
    <LI> Measure the concentration of DNA using Nanodrop (see Nanodrop protocol). </LI> <LI> Place tube in thermocycler, run standard golden gate protocol: 37C 5min, 50X: , 37 2.5min, 4C 0.5min, 16C 5.5min, 37C 10 min, 80C 20 min,
    41 KB (6,854 words) - 03:20, 29 October 2013
  • Team:SCUT/Project/Protocol
    <h1>Protocol</h1> ...rg/mediawiki/parts/9/98/Scut_Protocol_for_gene_knockout.pdf" class="brick">Protocol for gene knockout</a><br><br>
    3 KB (415 words) - 03:08, 28 September 2013
  • Team:UFMG Brazil/lab/protocols
    == Transformation Protocol (as suggested by iGEM-registry)== == PCR Protocol ==
    21 KB (2,780 words) - 13:02, 27 October 2013
  • Team:UFMG Brazil/Protocols
    == Transformation Protocol (as suggested by iGEM-registry)== == PCR Protocol ==
    15 KB (2,384 words) - 23:17, 28 October 2013
  • Team:Colombia Uniandes/Journal
    Harju et al., “Bust n’ Grab” Protocol for Yeast Genomic DNA Extraction ... tried extracting yeast genome using a modified Harju “Bust n’ Grab” protocol using three parallel methods:
    23 KB (3,678 words) - 02:30, 28 September 2013

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