Team:Hong Kong HKUST/experiment



Summary of experiments done this summer - The HKUST iGEM 2013 carried out a lot of experiments this summer, including regular plasmid construction, PCR, and mutagenesis. We listed the main experiments done under each module in a clear, forward way.

Experiment 1: Cell Viability & Fatty Acid Quantification

Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate

Experiment 2: Fatty Acid Sensing Mechanism

Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration

Experiment 3: Protein Trafficking

Target ACE proteins into mitochondria

Experiment 4: Glyoxylate Shunt

Introduce glyoxylate enzymes responsible for the shunt