Team:Valencia-CIPF/Strategy

From 2013.igem.org



Strategy

The strategy followed was to amplify the genes of the repellent and the aromas from the cDNA obtained from other laboratories by PCR and then clone them into E. coli under the control of different promoters and study its expression.

Afterwards, do the same in yeast.

To do this, some of the tools used were: DNA quantification by nanodrop, gel electrophoresis, plasmid extraction, production of competent cells, restriction enzyme digestions and cloning.