Team:Paris Saclay/Notebook/July/26

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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} ='''Notebook : August 12'''= =='''Lab work'''== ==='''A - Aerobic/Anaerobic regulation system'''=== ===='''Objective : obtaining .....")
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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining ...'''====
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===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006 ?????????????????'''====
===='''1 - Electrophoresis of PCR products : NarK, NarG, NirB'''====
===='''1 - Electrophoresis of PCR products : NarK, NarG, NirB'''====
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* Well 1 : 5µL of NirB+1µL of 6X loading dye
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* Well 1 : 5µL of NirB+1µL of 6X loading dye quantité 2µL d'ADN ????????
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* Well 2 : 5µL of NirB+1µL of 6X loading dye LA MEME CHOSE ???????
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* Well 2 : 5µL of NirB+1µL of 6X loading dye
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* Well 3 : 5µL of NarG+1µL of 6X loading dye
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* Well 3 : 5µL of NarK+1µL of 6X loading dye
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* Well 4 : 5µL of NarG+1µL of 6X loading dye
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* Well 4 : 5µL of NarK+1µL of 6X loading dye
* Well 5 : 6µL DNA Ladder
* Well 5 : 6µL DNA Ladder
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* Well 6 : 5µL of NarK+1µL of 6X loading dye NUMERO 4 ???????????????
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* Well 6 : 5µL of NarG+1µL of 6X loading dye  
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* Well 7 : 5µL of NarK+1µL of 6X loading dye
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* Well 7 : 5µL of NarG+1µL of 6X loading dye
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* Well 8 : 5µL of NarK+1µL of 6X loading dye  
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* Gel : 1.2%
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* Gel : 1%
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Expected sizes :
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* NarK, NarG, NirB : ...
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{|
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We obtain fragments at the right size. We can purify it.
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===='''4 - Gel purification of PCR products : NarK, NarG, NirB'''====
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Xavier
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Protocol : [[Team:Paris_Saclay/Protocols/Gel purification|Gel purification]]
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REPRIS dans 20µL !!!!!!!! ????????? PAS DE NANODROP ??????
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Revision as of 14:38, 11 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : August 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006 ?????????????????

1 - Electrophoresis of PCR products : NarK, NarG, NirB

Abdou

[[]]
  • Well 1 : 5µL of NirB+1µL of 6X loading dye
  • Well 2 : 5µL of NirB+1µL of 6X loading dye LA MEME CHOSE ???????
  • Well 3 : 5µL of NarG+1µL of 6X loading dye
  • Well 4 : 5µL of NarG+1µL of 6X loading dye
  • Well 5 : 6µL DNA Ladder
  • Well 6 : 5µL of NarK+1µL of 6X loading dye NUMERO 4 ???????????????
  • Well 7 : 5µL of NarK+1µL of 6X loading dye
  • Well 8 : 5µL of NarK+1µL of 6X loading dye
  • Gel : 1%

Expected sizes :

  • NarK, NarG, NirB : ...

We lost all our PCR products. We will do PCR again using more concentrated DNA and oligos.

2 - PCR of NarK, NarG, NirB

Abdou, Xavier

Used quantities : cf PCR précédente !!!!!!!!!!!!! ... ADN non dilué

PCR program : cf PCR précédente !!!!!!!!! SCHEMA !!!!!!!!!!!!!!

3 - Electrophoresis of PCR products : NarK, NarG, NirB

Abdou, Xavier

[[]]
  • Well 1 : 5µL of NirB+1µL of 6X loading dye quantité 2µL d'ADN ????????
  • Well 2 : 5µL of NirB+1µL of 6X loading dye
  • Well 3 : 5µL of NarK+1µL of 6X loading dye
  • Well 4 : 5µL of NarK+1µL of 6X loading dye
  • Well 5 : 6µL DNA Ladder
  • Well 6 : 5µL of NarG+1µL of 6X loading dye
  • Well 7 : 5µL of NarG+1µL of 6X loading dye
  • Gel : 1.2%

Expected sizes :

  • NarK, NarG, NirB : ...

We obtain fragments at the right size. We can purify it.

4 - Gel purification of PCR products : NarK, NarG, NirB

Xavier

Protocol : Gel purification

REPRIS dans 20µL !!!!!!!! ????????? PAS DE NANODROP ??????

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