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Team:Paris Saclay/Notebook/July/26
From 2013.igem.org
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006 | + | ===='''Objective : obtaining Bba_K1155003'''==== |
| + | |||
| + | ===='''1 - Ligation of RBS-AmilCP and Term in PSB1C3'''==== | ||
| + | |||
| + | Xavier | ||
| + | |||
| + | Used quantities : | ||
| + | |||
| + | * RBS-AmilCP : 2µL | ||
| + | * Bba_B0015 digested by ... : 2.5µL | ||
| + | * Buffer : 1µL | ||
| + | * Ligase : 1µL | ||
| + | * H2O : 3.5µL | ||
| + | |||
| + | ===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006'''==== | ||
===='''1 - Electrophoresis of PCR products : NarK, NarG, NirB'''==== | ===='''1 - Electrophoresis of PCR products : NarK, NarG, NirB'''==== | ||
Revision as of 15:28, 11 September 2013
Notebook : July 26
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Ligation of RBS-AmilCP and Term in PSB1C3
Xavier
Used quantities :
- RBS-AmilCP : 2µL
- Bba_B0015 digested by ... : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Electrophoresis of PCR products : NarK, NarG, NirB
Abdou
| [[]] |
|
Expected sizes :
- NarK, NarG, NirB : ...
|
We lost all our PCR products. We will do PCR again using more concentrated DNA and oligos. |
2 - PCR of NarK, NarG, NirB
Abdou, Xavier
Used quantities : cf PCR précédente !!!!!!!!!!!!! ... ADN non dilué
PCR program : cf PCR précédente !!!!!!!!! SCHEMA !!!!!!!!!!!!!!
3 - Electrophoresis of PCR products : NarK, NarG, NirB
Abdou, Xavier
| [[]] |
|
Expected sizes :
- NarK, NarG, NirB : ...
|
We obtain fragments at the right size. We can purify it. |
4 - Gel purification of PCR products : NarK, NarG, NirB
Xavier
Protocol : Gel purification
REPRIS dans 20µL !!!!!!!! ????????? PAS DE NANODROP ??????
