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Team:Paris Saclay/Notebook/August/2
From 2013.igem.org
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Revision as of 22:19, 15 September 2013
Contents |
Notebook : August 1
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Extraction of Bba_K1155003 from DH5α
Damir, Nadia
Protocol : Plasmid extraction
We used colony 9, 11 and 12. ???????? Elué dans 50µL d'H2O.
2 - Electrophoresis of the extraction of Bba_K1155003
Damir, Nadia
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Expected sizes :
- Bba_K1155003 : 2734 pb
Estimated concentrations :
- Clone 9 : 18ng/µL
- Clone 11 :18ng/µL
- Clone 12 : 18ng/µL
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We obtain fragment at the right size (Clone 12 starts 5 min after the others). The extraction was good. |
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
??????????
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Tranformation of BphR2, FNR and RBS-FNR in DH5α
XiaoJing
Protocol : Bacterial transformation
