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Team:Paris Saclay/Notebook/August/5
From 2013.igem.org
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ====''' | + | ===='''Objective : obtaining Bba_K1155003'''==== |
| - | Digestion of | + | ===='''1 - Digestion of Bba_K1155003''==== |
Nadia, XiaoJing | Nadia, XiaoJing | ||
| - | * | + | Used quantities : |
| + | * Bba_K1155003 : 5µL | ||
| + | * EcoRI FD : 1µL | ||
| + | * PstI FD : 1µL | ||
* Buffer FD : 3µL | * Buffer FD : 3µL | ||
| - | * | + | * H2O : 20µL |
| - | + | ||
| - | + | ||
| - | + | ||
| + | We let the digestion at 37°C. | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 22:29, 15 September 2013
Contents |
Notebook : August 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
'1 - Digestion of Bba_K1155003
Nadia, XiaoJing
Used quantities :
- Bba_K1155003 : 5µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- Buffer FD : 3µL
- H2O : 20µL
We let the digestion at 37°C.
