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Team:Paris Saclay/Notebook/August/5
From 2013.igem.org
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| Line 9: | Line 9: | ||
===='''Objective : obtaining Bba_K1155003'''==== | ===='''Objective : obtaining Bba_K1155003'''==== | ||
| - | ===='''1 - Digestion of Bba_K1155003''==== | + | ===='''1 - Digestion of Bba_K1155003'''==== |
Nadia, XiaoJing | Nadia, XiaoJing | ||
| Line 20: | Line 20: | ||
* H2O : 20µL | * H2O : 20µL | ||
| - | We let the digestion at 37°C | + | We let the digestion at 37°C during 10 minutes ?????? |
| + | |||
| + | ===='''2 - Electrophoresis of the digestion of Bba_K1155003'''==== | ||
| + | |||
| + | Damir | ||
| + | |||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 22:36, 15 September 2013
Contents |
Notebook : August 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Digestion of Bba_K1155003
Nadia, XiaoJing
Used quantities :
- Bba_K1155003 : 5µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- Buffer FD : 3µL
- H2O : 20µL
We let the digestion at 37°C during 10 minutes ??????
2 - Electrophoresis of the digestion of Bba_K1155003
Damir
