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Team:Paris Saclay/Notebook/August/5
From 2013.igem.org
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===='''Objective : obtaining Bba_K1155003'''==== | ===='''Objective : obtaining Bba_K1155003'''==== | ||
| - | ===='''1 - Digestion of Bba_K1155003'''==== | + | ===='''1 - Digestion of Bba_K1155003 by EcoRI/PstI'''==== |
Nadia, XiaoJing | Nadia, XiaoJing | ||
| Line 26: | Line 26: | ||
Damir | Damir | ||
| - | + | {| | |
| + | | style="width:350px;border:1px solid black;" |File:Ps.jpg|350px]] | ||
| + | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| + | * Well 1 : | ||
| + | * Well 2 : 6µL of DNA Ladder | ||
| + | * Well 3 : 30µL of Bba_K1155003 digested by EcoRI/PstI+6µl of 6X loading dye | ||
| + | * Well 4 : | ||
| + | * Well 5 : | ||
| + | * Well 6 : | ||
| + | * Well 7 : | ||
| + | * Well 8 : | ||
| + | * Gel : 1% | ||
| + | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 22:39, 15 September 2013
Contents |
Notebook : August 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Digestion of Bba_K1155003 by EcoRI/PstI
Nadia, XiaoJing
Used quantities :
- Bba_K1155003 : 5µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- Buffer FD : 3µL
- H2O : 20µL
We let the digestion at 37°C during 10 minutes ??????
2 - Electrophoresis of the digestion of Bba_K1155003
Damir
| File:Ps.jpg|350px]] |
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