Team:TU-Delft/Planning

From 2013.igem.org

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<a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">Kill Switch</font></a><br>
 
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<a href="https://2013.igem.org/Team:TU-Delft/TimerPlanning" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">Timer</font></a>  <br>
 
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<a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">All</font></a> <br>
 
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy related to each module can be viewed in the following links:
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<li><a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">Kill Switch</font></a></li>
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows:  
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<li><a href="https://2013.igem.org/Team:TU-Delft/TimerPlanning" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">Timer</font></a></li>
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<li><a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">All</font></a> </li>
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<h4>His 6 - SUMO Peptide construct </h4> <center>
 
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<img src="https://static.igem.org/mediawiki/2013/4/47/His6-SUMOPeptide-construct.jpg" height=800px width=680px ></center>
 
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<h4>Peptide Production</h4> <center>
 
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<img src="https://static.igem.org/mediawiki/2013/3/30/Peptide-Production.jpg" height=600px width=680px ></center>
 
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<h4>AIP test Construct </h4><center>
 
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<img src="https://static.igem.org/mediawiki/2013/d/d4/AIP-test-constructs.jpg" height=950px width=700px ></center>
 
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<h4> </h4> <center>
 
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<img src="  " height=950px width=680px ></center>
 
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Revision as of 11:22, 17 September 2013


Planning

Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy related to each module can be viewed in the following links: