Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

From 2013.igem.org

(Difference between revisions)
Line 34: Line 34:
- Prepared RNase for future use.
- Prepared RNase for future use.
-
- Prepared W3110 and BL21 cultures.
+
- Prepared T4 and T7 cultures.
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]

Revision as of 20:33, 12 June 2013


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Overview
March-April
May-June
July-August
September-October

6/10/13

- Prepared RNase for future use.

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/10/13

- Prepared RNase for future use.

- Prepared W3110 and BL21 cultures.


6/3/13

- Ran the CsCl purification step.

6.3 CsCl Gradient Phage Purification

- Next class we will attempt extracting the phage from the gradient.


6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6.5 Bacteria Cultures


6/7/13

Made 1 L of 10 X phage suspension buffer.
6.7 Phage Suspension Buffer Preparation

Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Dailylog"