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Team:Paris Saclay/Notebook/August/20
From 2013.igem.org
(Difference between revisions)
| Line 24: | Line 24: | ||
Used quantities : | Used quantities : | ||
| - | * | + | * Oligo 54F : 2µL |
| + | * Oligo 55R : 2µL | ||
| + | * DNA : 1µL | ||
| + | * Buffer Phusion : 10µL | ||
| + | * dNTP : 1µL | ||
| + | * Phusion : 1µL | ||
| + | * DMS9 : 2µL ?????????????????????????????????? | ||
| + | * H2O : 31µL | ||
| - | PCR | + | PCR program : |
| + | |||
| + | [[File:Pstest.jpg|400px]] | ||
===='''2 - Electrophoresis of PCR products : BphR2 Part I'''==== | ===='''2 - Electrophoresis of PCR products : BphR2 Part I'''==== | ||
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | + | We lost our fragment. We will do the PCR again. | |
|} | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 19:04, 20 September 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Sequences analysis
Damir, XiaoJing
...
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - PCR of BphR2 Part I
Damir
Used quantities :
- Oligo 54F : 2µL
- Oligo 55R : 2µL
- DNA : 1µL
- Buffer Phusion : 10µL
- dNTP : 1µL
- Phusion : 1µL
- DMS9 : 2µL ??????????????????????????????????
- H2O : 31µL
PCR program :
2 - Electrophoresis of PCR products : BphR2 Part I
Nadia
| [[]] |
|
|
We obtain a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : Gel purification
Nanodrop :
- BphR2 Part I: .........
|
We lost our fragment. We will do the PCR again. |
