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-	- Prepared RNase for future use.	+	- Began first step of purification process.
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-	- Prepared W3110 and BL21 cultures.	+
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Revision as of 20:03, 14 June 2013

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• iGEM 2013

Phage Purification May - June Notebook: June 10 - June 23 Daily Log

Overview March-April May-June July-August September-October

<< Previous Next >> 6/10/13 - Prepared RNase A for future use. 6.10 RNase A Preparation - Prepared T4 and T7 cultures. 6.10 Phage Preparation 6/12/13 - Began first step of purification process. 6/3/13 - Ran the CsCl purification step. 6.3 CsCl Gradient Phage Purification - Next class we will attempt extracting the phage from the gradient. 6/5/13 - Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle. Only extracted T4 because the T7 gradient was destroyed in the refrigerator. Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed. Placed these in the refrigerator. - Prepared stocks of bacteria for future use. 6.5 Bacteria Cultures 6/7/13 Made 1 L of 10 X phage suspension buffer. 6.7 Phage Suspension Buffer Preparation << Previous Next >>