Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

From 2013.igem.org

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<font size="4"> '''6/10/13''' </font>
 
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- Prepared RNase A for future use.
 
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]]
 
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- Prepared T4 and T7 cultures.
 
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]
 
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<font size="4"> '''6/12/13''' </font>
 
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
 
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<font size="4"> '''6/3/13''' </font>
<font size="4"> '''6/3/13''' </font>
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: Made 1 L of 10 X phage suspension buffer.
: Made 1 L of 10 X phage suspension buffer.
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
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<font size="4"> '''6/10/13''' </font>
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- Prepared RNase A for future use.
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 +
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]]
 +
 +
- Prepared T4 and T7 cultures.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]
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<br>
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<font size="4"> '''6/12/13''' </font>
 +
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
 +
 +
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
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Revision as of 20:46, 14 June 2013


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Overview
March-April
May-June
July-August
September-October


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6/3/13

- Ran the CsCl purification step.

6.3 CsCl Gradient Phage Purification

- Next class we will attempt extracting the phage from the gradient.


6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6.5 Bacteria Cultures


6/7/13

Made 1 L of 10 X phage suspension buffer.
6.7 Phage Suspension Buffer Preparation

6/10/13

- Prepared RNase A for future use.

6.10 RNase A Preparation

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.

6.12 PEG Purification



<< Previous Next >>


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