Team:Paris Saclay/Notebook/July/12

From 2013.igem.org

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==''summary''==
==''summary''==
For fnr regulator system:
For fnr regulator system:
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*performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
+
*Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
-
*design of oligo for amplifying AmiCP+RBS.
+
*Design of oligo for amplifying AmiCP+RBS.
For PCBs sensor system:
For PCBs sensor system:
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*had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
+
*Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
-
*plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
+
*Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
=='''Lab work'''==
=='''Lab work'''==

Revision as of 02:16, 22 September 2013

Notebook : July 12

summary

For fnr regulator system:

  • Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
  • Design of oligo for amplifying AmiCP+RBS.

For PCBs sensor system:

  • Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
  • Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.

Lab work

constructing


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