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Team:British Columbia/Notebook/Protocols
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===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]=== | ===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]=== | ||
Phosphorylate oligonucleotides prior to ligation | Phosphorylate oligonucleotides prior to ligation | ||
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| + | ===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells|Electrocompetent Cell Production]]=== | ||
| + | Make electrocompetent cells. | ||
Revision as of 01:10, 23 September 2013
iGEM Home
Contents |
Protocols
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
Electrocompetent Cell Production
Make electrocompetent cells.
