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From 2013.igem.org

Revision as of 20:36, 18 August 2013 (view source) FrancesKR (Talk | contribs) ← Older edit		Revision as of 01:47, 23 September 2013 (view source) AnnaMüller (Talk | contribs) Newer edit →
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	Decoy:		Decoy:
	*PAM + spacer (on Amp)		*PAM + spacer (on Amp)
		+
		+	=='''September 22nd'''==
		+
		+	'''Experimenter''': Anna Müller
		+
		+	'''Aim:''' Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.
		+
		+	'''Results:''' will follow.
		+
		+
		+	'''Aim:''' Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.
		+
		+	'''Results:''' will follow.

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Revision as of 01:47, 23 September 2013

iGEM Home

Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

• Cas9 - Joe and Anna
• pTet -
• const. promoter + Cas9 - Joe
• promoter + Cas9 + Leader + R + spacer + R (on Kan)
• L + R + spacer + R

Target:

• tracerRNA
• repeat + spacer + repeat - Vanins*2 + underlings
• leader - Ray, Fisal, Dan

Decoy:

• PAM + spacer (on Amp)

September 22nd

Experimenter: Anna Müller

Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.

Results: will follow.

Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.

Results: will follow.