23/09/13

From 2013.igem.org

Revision as of 09:24, 24 September 2013

Gel Results from the digestion performed on the 20/09/13

Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);

• The gel shows that digestions were successful as every sample dropped the inserted gene.

Gel Order:

F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).

• Same result as the gel above

Evaporation of isolated plasmids and re-suspended in 1xTE

• The samples were evaporated with a vacuum evaporator.
• The samples were re-suspended in 20 ul of 1xTE.