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23/09/13
From 2013.igem.org
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==Gel Results from the digestion performed on the [[20/09/13]]== | ==Gel Results from the digestion performed on the [[20/09/13]]== | ||
[[File:doubledigestion_biobrick_pSB1C3_230913.jpg]] | [[File:doubledigestion_biobrick_pSB1C3_230913.jpg]] | ||
| + | |||
Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P); | Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P); | ||
| + | |||
| + | *The gel shows that digestions were successful as every sample dropped the inserted gene. | ||
| + | |||
| + | [[File:igem_BB_230913.jpg]] | ||
| + | |||
| + | Gel Order: | ||
| + | |||
| + | F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P). | ||
| + | |||
| + | *Same result as the gel above | ||
| + | |||
| + | ==Evaporation of isolated plasmids and re-suspended in 1xTE== | ||
| + | |||
| + | *The samples were evaporated with a vacuum evaporator. | ||
| + | *The samples were re-suspended in 20 ul of 1xTE. | ||
| + | |||
| + | {|border=1 | ||
| + | |Sample||Volume (ul)||Concentration (ng/ul)||280/260||260/230 | ||
| + | |- | ||
| + | |B4||20||12.1||1.49||1.44 | ||
| + | |- | ||
| + | |B9||20||16.3||1.63||1.45 | ||
| + | |- | ||
| + | |B12||20||26.3||1.87||1.05 | ||
| + | |- | ||
| + | |X3||20||27.3||1.78||1.11 | ||
| + | |- | ||
| + | |X5||20||15.6||1.6||1.35 | ||
| + | |- | ||
| + | |X6||20||25.2||1.63||1.46 | ||
| + | |- | ||
| + | |F1||20||38.9||1.83||1.57 | ||
| + | |- | ||
| + | |F10||20||33.6||1.67||1.4 | ||
| + | |- | ||
| + | |F12||20||6.5||1.48||1.10 | ||
| + | |} | ||
| + | |||
| + | ==Luria Agar plates== | ||
| + | |||
| + | *Luria agar plates were made for the plating of the CSE kits that arrived. | ||
Latest revision as of 09:26, 24 September 2013
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Gel Results from the digestion performed on the 20/09/13
Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);
- The gel shows that digestions were successful as every sample dropped the inserted gene.
Gel Order:
F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).
- Same result as the gel above
Evaporation of isolated plasmids and re-suspended in 1xTE
- The samples were evaporated with a vacuum evaporator.
- The samples were re-suspended in 20 ul of 1xTE.
| Sample | Volume (ul) | Concentration (ng/ul) | 280/260 | 260/230 |
| B4 | 20 | 12.1 | 1.49 | 1.44 |
| B9 | 20 | 16.3 | 1.63 | 1.45 |
| B12 | 20 | 26.3 | 1.87 | 1.05 |
| X3 | 20 | 27.3 | 1.78 | 1.11 |
| X5 | 20 | 15.6 | 1.6 | 1.35 |
| X6 | 20 | 25.2 | 1.63 | 1.46 |
| F1 | 20 | 38.9 | 1.83 | 1.57 |
| F10 | 20 | 33.6 | 1.67 | 1.4 |
| F12 | 20 | 6.5 | 1.48 | 1.10 |
Luria Agar plates
- Luria agar plates were made for the plating of the CSE kits that arrived.
