23/09/13

From 2013.igem.org

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(Gel Results from the digestion performed on the 20/09/13)
 
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[[File:igem_BB_230913.jpg]]
[[File:igem_BB_230913.jpg]]
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Gel Order:
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F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).
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*Same result as the gel above
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==Evaporation of isolated plasmids and re-suspended in 1xTE==
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*The samples were evaporated with a vacuum evaporator.
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*The samples were re-suspended in 20 ul of 1xTE.
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{|border=1
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|Sample||Volume (ul)||Concentration (ng/ul)||280/260||260/230
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|-
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|B4||20||12.1||1.49||1.44
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|-
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|B9||20||16.3||1.63||1.45
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|-
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|B12||20||26.3||1.87||1.05
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|-
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|X3||20||27.3||1.78||1.11
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|-
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|X5||20||15.6||1.6||1.35
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|-
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|X6||20||25.2||1.63||1.46
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|-
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|F1||20||38.9||1.83||1.57
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|-
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|F10||20||33.6||1.67||1.4
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|-
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|F12||20||6.5||1.48||1.10
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|}
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==Luria Agar plates==
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*Luria agar plates were made for the plating of the CSE kits that arrived.

Latest revision as of 09:26, 24 September 2013

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Gel Results from the digestion performed on the 20/09/13

Doubledigestion biobrick pSB1C3 230913.jpg

Gel Order: X3 (E/P), X3 (X/P); X5 (E/P), X5 (X/P); X6 (E/P), X6 (X/P); X12 (E/P), X12 (X/P); F1 (E/P), F1 (X/P);

  • The gel shows that digestions were successful as every sample dropped the inserted gene.

Igem BB 230913.jpg

Gel Order:

F8 (E/P), F8 (X/P); F10 (E/P), F10 (X/P); F12 (E/P), F12 (X/P); B4 (E/P), B4 (X/P); B5 (E/P), B5 (X/P); B9 (E/P), B9 (X/P); B12 (E/P), B12 (X/P).

  • Same result as the gel above

Evaporation of isolated plasmids and re-suspended in 1xTE

  • The samples were evaporated with a vacuum evaporator.
  • The samples were re-suspended in 20 ul of 1xTE.
SampleVolume (ul)Concentration (ng/ul)280/260260/230
B42012.11.491.44
B92016.31.631.45
B122026.31.871.05
X32027.31.781.11
X52015.61.61.35
X62025.21.631.46
F12038.91.831.57
F102033.61.671.4
F12206.51.481.10

Luria Agar plates

  • Luria agar plates were made for the plating of the CSE kits that arrived.