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Team:BYU Provo/Notebook/Cholera - Enzymes/July-August
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| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We focused these two weeks on preparing our DspB and Savinase enzymes for protein purification so we could test them in our Biofilm Assay. We ran PCRs, restriction digests, ligations, and transformations on them.</font> |
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| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We checked the transformation products from our earlier work with colony PCR, but the transformations were unsuccessful. We worked this period on trying to get our ligations and transformations to work.</font> |
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| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We continued working on getting DspB and Savinase ready for protein purification. We were having so many problems with Savinase that we went back and prepared new template DNA from ''Bacillus subtilis'' to use for our Savinase enzyme. </font> |
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Revision as of 01:42, 25 September 2013
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July 1 - July 14
We continued to have issues with washing our biofilm during the biofilm assay without losing significant portions of the biofilm. We tried several different methods to try to find the best method for treating V. cholerae biofilm specifically. After several different approaches, we found that the most consistent way to treat the biofilm during the assay is to pellet and resuspend the biofilm with each wash, allowing us to wash our sample without losing the biofilm itself.
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| July 15 - July 31
We focused these two weeks on preparing our DspB and Savinase enzymes for protein purification so we could test them in our Biofilm Assay. We ran PCRs, restriction digests, ligations, and transformations on them.
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| August 1 - August 14
We checked the transformation products from our earlier work with colony PCR, but the transformations were unsuccessful. We worked this period on trying to get our ligations and transformations to work.
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| August 15 - August 31
We continued working on getting DspB and Savinase ready for protein purification. We were having so many problems with Savinase that we went back and prepared new template DNA from Bacillus subtilis to use for our Savinase enzyme.
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