Revision as of 14:09, 25 September 2013

Transformation

We suspended the DNA with water from iGEM plate and performed several transformation.

BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
Simple terminator BBa_B0010 into plasmid PSB1A2.

Navigation Home Team Project Labwork Notebook Human practices

Notebook : July 12

Lab work

'A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010

Zhou

Transformation of 07/10/13 of Bba_B0010 and Bba_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

1 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2

Zhou

Used quantities :

Glycerol 85% : 425
Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BphA1 and BphR2

Anaïs

We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.

Previous day

Back to calendar

Next week

@@ Line 1: / Line 1: @@
-{{Team:Paris_Saclay/incl_debut_generique}}
-='''Notebook : July 12'''=
-==''summary''==
-For fnr regulator system:
-*Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
-*Design of oligo for amplifying AmiCP+RBS.
-For PCBs sensor system:
-*Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
-*Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
-=='''Lab work'''==
-A.aero/anaerobic regulation system:
-*BioBrick RBS+LacZ+terminator in plasmid PSB1C3
-<br>
 <u>Transformation</u>
@@ Line 23: / Line 9: @@
 *Simple terminator BBa_B0010 into plasmid PSB1A2.
-see protocol Tranformation.
-B.PCBs sensor system:
-<p>The stock and extraction of promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 performed according to protocol. Please see protocol DNA extraction and sample stock.</p>
-<br>
+{{Team:Paris_Saclay/incl_debut_generique}}
+='''Notebook : July 12'''=
+=='''Lab work'''==
+==='''A - Aerobic/Anaerobic regulation system''===
+===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
+===='''1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010'''====
+Zhou
+{|
+| style="border:1px solid black;padding:5px;background-color:#DE;" |
+Transformation of 07/10/13 of Bba_B0010 and Bba_I732019 didn't works. We will do it again.
+|}
+Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
+===='''1 -Design of RBS-AmilCP oligos'''====
+Abdou, Anaïs
+We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
+==='''B - PCB sensor system'''===
+===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''====
+===='''1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2'''====
+Zhou
+Used quantities :
+* Glycerol 85% : 425
+* Culture : 1µL
+===='''2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α'''====
+Abdou, Sheng
+Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
+===='''3 - Streak of BphA1 and BphR2'''====
+Anaïs
+We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
 {| border="1" align="center"
 |[[Team:Paris Saclay/Notebook/July/11|<big>Previous day</big>]]
@@ Line 36: / Line 81: @@
 |<big>Next week</big>
 |}
 {{Team:Paris_Saclay/incl_fin}}

Team:Paris Saclay/Notebook/July/12

From 2013.igem.org