Team:Paris Saclay/Notebook/July/12

From 2013.igem.org

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<u>Transformation</u>
 
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<p>We suspended the DNA with water from iGEM plate and performed several transformation.</p><br>
 
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*BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
 
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*BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
 
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*BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
 
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*Simple terminator BBa_B0010 into plasmid PSB1A2.
 
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{{Team:Paris_Saclay/incl_debut_generique}}
{{Team:Paris_Saclay/incl_debut_generique}}
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=='''Lab work'''==
=='''Lab work'''==
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==='''A - Aerobic/Anaerobic regulation system''===
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==='''A - Aerobic/Anaerobic regulation system'''===
===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
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Transformation of 07/10/13 of Bba_B0010 and Bba_I732019 didn't works. We will do it again.
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Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. We obtain two colonies of 07/10/13 transformation. We will extract Bba_B0010 and do the transformation again.
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Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
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===='''1 -Design of RBS-AmilCP oligos'''====
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===='''2 -Design of RBS-AmilCP oligos'''====
Abdou, Anaïs
Abdou, Anaïs
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
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===='''3 -Extraction of Bba_B0010 from DH5α'''====
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Sheng
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Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]

Revision as of 14:12, 25 September 2013

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Contents

Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010

Zhou

Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. We obtain two colonies of 07/10/13 transformation. We will extract Bba_B0010 and do the transformation again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

3 -Extraction of Bba_B0010 from DH5α

Sheng

Protocol : High copy plamid extraction


B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2

Zhou

Used quantities :

  • Glycerol 85% : 425
  • Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BphA1 and BphR2

Anaïs

We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.

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