"
Team:TU-Eindhoven/Production
From 2013.igem.org
(→Protein Production) |
JacquesErnes (Talk | contribs) (→Protein Production) |
||
| Line 10: | Line 10: | ||
| - | * Poly Arginine-Glycine * Human Protomine 1 E. coli opt | + | * Poly Arginine-Glycine |
| - | * 1G70 * 1PJN | + | * Human Protomine 1 E. coli opt |
| - | * 1ETF * Poly Lysine-Serine | + | * 1G70 |
| - | * Poly Arginine-Serine * Poly Threonine-Lysine | + | * 1PJN |
| - | * Poly Arginine-Threonine * Poly Arginine | + | * 1ETF |
| + | * Poly Lysine-Serine | ||
| + | * Poly Arginine-Serine | ||
| + | * Poly Threonine-Lysine | ||
| + | * Poly Arginine-Threonine | ||
| + | * Poly Arginine | ||
| - | + | Some of these proteins were selected using the RCSB Protein Data Bank, a python program designed by us and a molecular dynamics simulations, to ensure that the selected proteins had a high content of Arginine and Lysine. The complete description of the protein selection process can be found on the [[Team:TU-Eindhoven/ProteinSelection| protein selection section of the drylab tab.]] The rest of the proteins were selected using the results of McMahon's research (2008) [1]. | |
| + | |||
| + | == Resources == | ||
| + | $$McMahon, M.T., 2008, New “Multi-Color” Polypeptide DIACEST Contrast Agents for MR Imaging, Magn Reson Med. 2008 October ; 60(4): 803–812.$$ | ||
Revision as of 20:15, 25 September 2013
Protein Production
The protein production is induced by the FNR promoter. 1 of the 10 different proteins shown below is generated by the device when a hypoxic environment is sensed. These proteins are rich in Arginine and Lysine, which are known to have good CEST qualities.
The proteins that the CEST proteins production device can generate are the following:
- Poly Arginine-Glycine
- Human Protomine 1 E. coli opt
- 1G70
- 1PJN
- 1ETF
- Poly Lysine-Serine
- Poly Arginine-Serine
- Poly Threonine-Lysine
- Poly Arginine-Threonine
- Poly Arginine
Some of these proteins were selected using the RCSB Protein Data Bank, a python program designed by us and a molecular dynamics simulations, to ensure that the selected proteins had a high content of Arginine and Lysine. The complete description of the protein selection process can be found on the protein selection section of the drylab tab. The rest of the proteins were selected using the results of McMahon's research (2008) [1].
Resources
$$McMahon, M.T., 2008, New “Multi-Color” Polypeptide DIACEST Contrast Agents for MR Imaging, Magn Reson Med. 2008 October ; 60(4): 803–812.$$
