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Team:TU-Eindhoven/Production
From 2013.igem.org
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=Protein Production= | =Protein Production= | ||
| - | Protein production is induced by the FNR promoter. | + | Protein production is induced by the FNR promoter. Each of the 8 proteins listed below can be expressed once a hypoxic environment is sensed. These proteins are all rich in Arginine and Lysine amino acids, which are known to be good CEST candidates. |
| - | The proteins that the CEST | + | The proteins that the CEST protein production device can generate are: |
| - | + | ||
| - | + | * Human Protomine 1 Optimized | |
| - | + | * 1ETF | |
| - | * Human Protomine 1 | + | * 1G70 |
| - | * 1G70 | + | |
* 1PJN | * 1PJN | ||
| - | * | + | * Poly Arginine-Glycine |
| + | * Poly Arginine-Serine | ||
| + | * Poly Threonine-Lysine | ||
* Poly Lysine-Serine | * Poly Lysine-Serine | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | Some of these proteins were selected using the RCSB Protein Data Bank | + | Some of these proteins were selected using the RCSB Protein Data Bank. A python program was designed by us alongside a molecular dynamics simulator, to ensure that the selected proteins had a high content of Arginine and Lysine aminoacids. The complete description of the protein selection process can be found on the [[Team:TU-Eindhoven/ProteinSelection| protein selection section of the drylab tab.]] The rest of the proteins were selected using the results of McMahon's research (2008) [1]. |
== References == | == References == | ||
Revision as of 10:30, 26 September 2013
Protein Production
Protein production is induced by the FNR promoter. Each of the 8 proteins listed below can be expressed once a hypoxic environment is sensed. These proteins are all rich in Arginine and Lysine amino acids, which are known to be good CEST candidates.
The proteins that the CEST protein production device can generate are:
- Human Protomine 1 Optimized
- 1ETF
- 1G70
- 1PJN
- Poly Arginine-Glycine
- Poly Arginine-Serine
- Poly Threonine-Lysine
- Poly Lysine-Serine
Some of these proteins were selected using the RCSB Protein Data Bank. A python program was designed by us alongside a molecular dynamics simulator, to ensure that the selected proteins had a high content of Arginine and Lysine aminoacids. The complete description of the protein selection process can be found on the protein selection section of the drylab tab. The rest of the proteins were selected using the results of McMahon's research (2008) [1].
References
[1] McMahon, M.T., 2008, New “Multi-Color” Polypeptide DIACEST Contrast Agents for MR Imaging, Magn Reson Med. 2008 October ; 60(4): 803–812.
