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Team:Paris Saclay/Notebook/July/12
From 2013.igem.org
(Difference between revisions)
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===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''==== | ===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''==== | ||
| - | ===='''1 - Stock of clones 5, 6, 7, 8 for | + | ===='''1 - Stock of clones 5, 6, 7, 8 for Bba_K1155002, clones 5, 6 for Bba_K1155001 and clones 3, 4 for BphR2'''==== |
Zhou | Zhou | ||
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* Culture : 1µL | * Culture : 1µL | ||
| - | ===='''2 - Extraction of clones 5, 6, 7, 8 for | + | ===='''2 - Extraction of clones 5, 6, 7, 8 for Bba_K1155002, clones 5, 6 for Bba_K1155001 and clones 3, 4 for BphR2 from DH5α'''==== |
Abdou, Sheng | Abdou, Sheng | ||
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Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] | Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] | ||
| - | ===='''3 - Streak of | + | ===='''3 - Streak of Bba_K1155002 and BphR2'''==== |
Anaïs | Anaïs | ||
Revision as of 15:45, 26 September 2013
Notebook : July 12
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017
Zhou
|
Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. |
Protocol : Bacterial transformation
2 -Design of RBS-AmilCP oligos
Abdou, Anaïs
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
B - PCB sensor system
Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2
1 - Stock of clones 5, 6, 7, 8 for Bba_K1155002, clones 5, 6 for Bba_K1155001 and clones 3, 4 for BphR2
Zhou
Used quantities :
- Glycerol 85% : 425
- Culture : 1µL
2 - Extraction of clones 5, 6, 7, 8 for Bba_K1155002, clones 5, 6 for Bba_K1155001 and clones 3, 4 for BphR2 from DH5α
Abdou, Sheng
Protocol : High copy plamid extraction
3 - Streak of Bba_K1155002 and BphR2
Anaïs
We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
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