Team:NYMU-Taipei/Project/Inhibition/Killing

Introduction

　　At first, we came up with several chemicals that might achieve our goal. Some of them are kinds of antimicrobial peptides, such as Apidaecin, [http://parts.igem.org/Part:BBa_K1104300 Abaecin], Hymenoptaecin, [http://parts.igem.org/Part:BBa_K1104301 Defensin1], which are all antimicrobial peptides in bees. Because these chemicals are secreted by bees themselves, they definitely do no harm to bees. Since we couldn’t find how to get the antimicrobial peptides, we finally decided to synthesize them by ourselves. Among four kinds of antimicrobial peptides, Apidaecin and Hymenoptaecin were much bigger so that it would be hard to synthesize, which was the first reason why we chose [http://parts.igem.org/Part:BBa_K1104300 Abaecin] and [http://parts.igem.org/Part:BBa_K1104301 Defensin1] to treat bees. The second reason was that the mRNA of Apidaecin and Hymenoptaecin couldn’t be found. In addition to antimicrobial peptides, we figured out other chemicals, which were Chitinase, Herein, Protofil, Cytochrome P450. Unfortunately, Chitinase, Herein, Protofil, and Cytochrome P450 have several defects, for example, they are all macromolecules, which make them difficult to be synthesized by E. coli. Moreover, even though E. coli successfully synthesize them, it is too hard to make these chemicals released. According to all of the above, we finally decided to use [http://parts.igem.org/Part:BBa_K1104301 Defensin1] and [http://parts.igem.org/Part:BBa_K1104300 Abaecin] to treat the bees infected by Nosema ceranae.