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Team:Paris Saclay/Notebook/July/16
From 2013.igem.org
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We let our digestion 1h30 at 37°C. | We let our digestion 1h30 at 37°C. | ||
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| + | ===='''2 - Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI'''==== | ||
| + | |||
| + | Sheng | ||
| + | |||
| + | {| | ||
| + | | style="width:350px;border:1px solid black;" |[[]] | ||
| + | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| + | * Well 1 : 5µL of PSB3K3 digested by EcoRI/PstI+1µL of 6X loading dye | ||
| + | * Well 2 : 5µL of PSB3K3 digested by EcoRI/PstI+1µL of 6X loading dye | ||
| + | * Well 3 : 6µL of DNA ladder | ||
| + | * Well 4 : 5µL of PSB3K3 digested by EcoRI/PstI+1µL of 6X loading dye | ||
| + | * Well 5 : 5µL of PSB3K3 digested by EcoRI/PstI+1µL of 6X loading dye | ||
| + | * Gel : 1% | ||
| + | |} | ||
| + | |||
| + | Expected sizes : | ||
| + | * PSB3K3 : 2750bp | ||
| + | |||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | We obtain fragments at the right size. | ||
| + | |} | ||
{| border="1" align="center" | {| border="1" align="center" | ||
Revision as of 16:33, 26 September 2013
Contents |
Notebook : July 16
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
Zhou
Protocol : High copy plamid extraction
Objective : obtaining biobricks in PSB3K3
1 - Digestion of PSB3K3 by EcoRI/PstI
Anaïs
Used quantities :
- DNA : 5µL
- Buffer FD : 2µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- H2O : 11µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
Sheng
| [[]] |
|
Expected sizes :
- PSB3K3 : 2750bp
|
We obtain fragments at the right size. |
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