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Team:Paris Saclay/Notebook/July/16
From 2013.igem.org
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* EcoRI FD : 1µL | * EcoRI FD : 1µL | ||
* PstI FD : 1µL | * PstI FD : 1µL | ||
| - | * H2O : | + | * H2O : 21µL |
We let our digestion 1h30 at 37°C. | We let our digestion 1h30 at 37°C. | ||
Revision as of 17:15, 26 September 2013
Notebook : July 16
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
Zhou
Protocol : High copy plamid extraction
1 - Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
Anaïs
- DNA : 5µL
- Buffer FD : 2µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- H2O : 21µL
We let our digestion 1h30 at 37°C.
Objective : obtaining biobricks in PSB3K3
1 - Digestion of PSB3K3 by EcoRI/PstI
Anaïs
Used quantities :
- DNA : 5µL
- Buffer FD : 2µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- H2O : 11µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
Sheng
| [[]] |
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Expected sizes :
- PSB3K3 : 2750bp
|
We obtain fragments at the right size. |
3 - Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
Abdou
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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