"
Team:USTC CHINA/Notebook/Protocols/Double Digestion
From 2013.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 7: | Line 7: | ||
<div class="bar" align="center"> | <div class="bar" align="center"> | ||
<div class="container" align="left"> | <div class="container" align="left"> | ||
| - | |||
| - | |||
<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div> | <div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div> | ||
| + | |||
<ul id="nav"> | <ul id="nav"> | ||
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> | |
| - | + | <ul class="subs"> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li> | |
| - | + | </ul> | |
| - | + | </li> | |
| - | + | <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a> | |
| - | + | <ul class="subs"> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li> | |
| - | + | </ul> | |
| - | + | </li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a> | |
| - | + | <ul class="subs"> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li> | |
| - | + | </ul> | |
| - | + | </li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a> | |
| - | + | <ul class="subs"> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li> | |
| - | + | </ul> | |
| - | + | </li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a> | |
| - | + | <ul class="subs"> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li> | |
| - | + | <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li> | |
| - | + | <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li> | |
| - | + | </ul> | |
| - | + | </li> | |
| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li> | |
| - | + | </ul> | |
| + | <div id="tlogo"><img src="https://static.igem.org/mediawiki/2013/f/f8/2013ustc-china_T-VACCINE.png" width="100%" height="123" /> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="content" align="center"> | <div class="content" align="center"> | ||
<div class="conbar1"> | <div class="conbar1"> | ||
| - | <div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> > <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook"> | + | <div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> > <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a> > <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a>> <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Double Digestion">Double Digestion</a></div></div> |
<div class="conbar2"> | <div class="conbar2"> | ||
<div class="leftbar" align="left"> | <div class="leftbar" align="left"> | ||
| Line 59: | Line 60: | ||
<h1>Double Digestion</h1> | <h1>Double Digestion</h1> | ||
<p>1.Performed with Thermo Scientific FastDigest. There are four restriction endonucleases we used in our experiments: EcoRI, XbaI, SpeI, PstI.</br> | <p>1.Performed with Thermo Scientific FastDigest. There are four restriction endonucleases we used in our experiments: EcoRI, XbaI, SpeI, PstI.</br> | ||
| - | <span> | + | <span>Double Digestion</span></br> |
<table width="580" border="2"> | <table width="580" border="2"> | ||
<tr> | <tr> | ||
| Line 90: | Line 91: | ||
</tr> | </tr> | ||
</table> | </table> | ||
| - | |||
| - | |||
2. Mix gently and spin down.</br> | 2. Mix gently and spin down.</br> | ||
3. Incubate at 37°C in a heat block or water thermostat for 30 min.</br> | 3. Incubate at 37°C in a heat block or water thermostat for 30 min.</br> | ||
4. Inactivate the enzyme by adding glycerol DNA loading buffer, or by heating for 5 min at 80°C(only for EcoRI) or 20min at 65°C(only for XbaI)</br> | 4. Inactivate the enzyme by adding glycerol DNA loading buffer, or by heating for 5 min at 80°C(only for EcoRI) or 20min at 65°C(only for XbaI)</br> | ||
| + | </p></div> | ||
| + | </div> | ||
| + | |||
<div class="rightbar"> | <div class="rightbar"> | ||
| - | <div class="port-sidebar-border"><h> | + | <div class="port-sidebar-border"><h>Notebook</h></div> |
<div class="clear"></div> | <div class="clear"></div> | ||
| - | <div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline"> | + | <div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></div> |
| - | <div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"> | + | <div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></div></div> |
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 08:56, 27 September 2013
