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Team:USTC CHINA/Notebook/Protocols/Constitutive promoter measurements
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<h1>Constitutive promoter measurements</h1> | <h1>Constitutive promoter measurements</h1> | ||
<p>1. Streak a LB plate of the strain.</br> | <p>1. Streak a LB plate of the strain.</br> | ||
| - | 2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic with single colony | + | 2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic with single colony from the plate.</br> |
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3. Cultures were grown in test tubes for 16hrs at 37℃ with shaking at 200rpm.</br> | 3. Cultures were grown in test tubes for 16hrs at 37℃ with shaking at 200rpm.</br> | ||
4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.</br> | 4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.</br> | ||
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Latest revision as of 09:17, 27 September 2013
