Team:NYMU-Taipei/Experiment/Wet Lab

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
{{:Team:NYMU-Taipei/Header}}
{{:Team:NYMU-Taipei/Header}}
-
==N. ceranae Experiment Flow==
+
==Coding Sequence Testing==
 +
We define the germination degree as follows:
 +
{|
 +
|-
 +
|[[File:Nymu-Ptps5 .png|thumb|250px|''' 1 point:A spore begins its budding reproduction.''']]||[[File:POINT 1.png|thumb|250px|''' 2 points:A new spore is ccompletely germinanated from the old one.''']]
 +
|}
 +
{|
 +
|-
 +
|[[File:Nymu-POINT 2.png|thumb|250px|''' 3 points:A cluster of duplicated spores is formed.''']]||[[File:Nymu-Tv10 ptp unsure.PNG|thumb|250px|''' 4 points:A Spore extrude a polar filament.''']]
 +
|}
 +
 
[[File:Nymu 流程圖.png|frame|center]]
[[File:Nymu 流程圖.png|frame|center]]
==N. ceranae Experiment Protocols==
==N. ceranae Experiment Protocols==
Line 12: Line 22:
[[File:Nymu-Germination.PNG|frame|center]]
[[File:Nymu-Germination.PNG|frame|center]]
-
==Coding Sequence Testing==
 
-
We define the germination degree as follows:
 
-
{|
 
-
|-
 
-
|[[File:Nymu-Ptps5 .png|thumb|250px|''' 1 point:A spore begins its budding reproduction.''']]||[[File:POINT 1.png|thumb|250px|''' 2 points:A new spore is ccompletely germinanated from the old one.''']]
 
-
|}
 
-
{|
 
-
|-
 
-
|[[File:Nymu-POINT 2.png|thumb|250px|''' 3 points:A cluster of duplicated spores is formed.''']]||[[File:Nymu-Tv10 ptp unsure.PNG|thumb|250px|''' 4 points:A Spore extrude a polar filament.''']]
 
-
|}
 

Revision as of 13:55, 27 September 2013

National Yang Ming University


Contents

Coding Sequence Testing

We define the germination degree as follows:

1 point:A spore begins its budding reproduction.
2 points:A new spore is ccompletely germinanated from the old one.
3 points:A cluster of duplicated spores is formed.
4 points:A Spore extrude a polar filament.
Nymu 流程圖.png

N. ceranae Experiment Protocols

Extraction of N. ceranae Spores from Bees

Extraction.png

Purification of N. ceranae Spores

Nymu-Purification.png

N. ceranae Genomic DNA Extraction

Nymu-Genomic.png

Germination of N. ceranae Spores

Nymu-Germination.PNG