Team:NYMU-Taipei/Experiment/Wet Lab

From 2013.igem.org

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{{:Team:NYMU-Taipei/Header}}
{{:Team:NYMU-Taipei/Header}}
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=N. ceranae experiment protocols=
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==Coding Sequence Testing==
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==Extraction of N. ceranae Spores from Bees==
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[[File:Nymu 流程圖.png|frame|center]]
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==N. ceranae Experiment Protocols==
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===Extraction of N. ceranae Spores from Bees===
[[File:Extraction.png|frame|center]]
[[File:Extraction.png|frame|center]]
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==Purification of N. ceranae Spores==
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===Purification of N. ceranae Spores===
[[File:Nymu-Purification.png|frame|center]]
[[File:Nymu-Purification.png|frame|center]]
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==N. ceranae Genomic DNA Extraction==
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===N. ceranae Genomic DNA Extraction===
[[File:Nymu-Genomic.png|frame|center]]
[[File:Nymu-Genomic.png|frame|center]]
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==Germination of N. ceranae Spores==
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===Germination of N. ceranae Spores===
[[File:Nymu-Germination.PNG|frame|center]]
[[File:Nymu-Germination.PNG|frame|center]]
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=Coding Sequence Testing=
 
We define the germination degree as follows:
We define the germination degree as follows:
{|
{|
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|[[File:Nymu-POINT 2.png|thumb|250px|''' 3 points:A cluster of duplicated spores is formed.''']]||[[File:Nymu-Tv10 ptp unsure.PNG|thumb|250px|''' 4 points:A Spore extrude a polar filament.''']]
|[[File:Nymu-POINT 2.png|thumb|250px|''' 3 points:A cluster of duplicated spores is formed.''']]||[[File:Nymu-Tv10 ptp unsure.PNG|thumb|250px|''' 4 points:A Spore extrude a polar filament.''']]
|}
|}
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And so far we have accomplished experiments of the first two groups, discovering that spores underwent the germination procesure got much more points than the control group. Next we will compare the points of group 3 and group 4 to see if Bee. coli can inhibit the germination of N. ceranae. 
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==Novel protocol for differentiation of N. apis and N. ceranae==
 
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''Nosema apis'' and ''Nosema ceranae'' are two genetically related microsporidian pathogens of the western honey bee or European honey bee (''Apis mellifera''). Both of them have similar life cycles, shorten the adult bee’s lifespan and impact honey production, but only ''N. ceranae'' is suspected to induce the CCD problem. Also, ''N. apis'' is well controlled by the use of fumagillin while ''N. ceranae'' escapes the antimicrobial agent. (http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003185) In recent years, ''N. ceranae'', originally found in the Asian honey bee (''Apis cerana''), appears to have become more dominant than ''N. apis'' in the western honey bees. The distinction between ''N. apis'' and ''N. ceranae'' is thus important and still worth further research.
 
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Light microscopy method based on morphology revealed that spores of ''N. ceranae'' were oval or rod shaped like a rice, and were slightly thinner than ''N. apis''.
 
{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Revision as of 14:14, 27 September 2013

National Yang Ming University


Contents

Coding Sequence Testing

Nymu 流程圖.png

N. ceranae Experiment Protocols

Extraction of N. ceranae Spores from Bees

Extraction.png

Purification of N. ceranae Spores

Nymu-Purification.png

N. ceranae Genomic DNA Extraction

Nymu-Genomic.png

Germination of N. ceranae Spores

Nymu-Germination.PNG

We define the germination degree as follows:

1 point:A spore begins its budding reproduction.
2 points:A new spore is ccompletely germinanated from the old one.
3 points:A cluster of duplicated spores is formed.
4 points:A Spore extrude a polar filament.

And so far we have accomplished experiments of the first two groups, discovering that spores underwent the germination procesure got much more points than the control group. Next we will compare the points of group 3 and group 4 to see if Bee. coli can inhibit the germination of N. ceranae.


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