Team:Hong Kong HKUST/results

From 2013.igem.org

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<div id="slide"><center><br><h3 class="title">Result</h3></center><br>
<div id="slide"><center><br><h3 class="title">Result</h3></center><br>
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<font size=3><p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
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<p id="yo"><font size=3>Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
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   <li>Successfully constructed the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li>
   <li>Successfully constructed the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li>
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   <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li>
   <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li>
   <li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li>
   <li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li>
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Revision as of 16:01, 27 September 2013





Result


Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:

  • Successfully constructed the two parts for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (BBa_K1119000, BBa_K1119001).
  • Successfully constructed the part of aceA and aceB for in glyoxylate shunt (BBa_K1119002, BBa_K1119003).
  • Successfully constructed the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (BBa_K1119004).
  • Successfully construct the part for a constitutive CMV promoter (BBa_K1119006).
  • Successfully characterized the regulatory promoter pCMV in HEK293FT cells (BBa_K1119008).
  • Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (BBa_K1119009).
  • Successfully tested the HepG2 cell viability in different sodium palmitate concentrations.



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