Team:Hong Kong HKUST/data

This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click here to access our result page for an overview of our achievements with those parts.

How our system works:

Data for Our Favorite New Parts:

• Mitochondria leader sequence, BBa_K1119001 : for directing protein into mitochondria
• In the presence of mitochondria leader sequence, glyoxylate enzymes AceA and AceB can be led into mitochondria to introduce glyoxylate shunt.
• Cytomegalovirus promoter (CMV Promoter), BBa_K1119006: constitutive mammalian promoter
• With the CMV promoter, glyoxylate genes aceA and aceB can be driven to be expressed.

Data for Pre-existing Parts:

• Kozak-XbaI-NLS-6His-stop, BBa_J176171 (Haynes Lab, 2007):  used as a backbone for PfadBA and Pfabp1.
• hGH-PolyA, BBa_K404108 (Freiburg Bioware iGEM, 2010):  hGH PolyA tail was added after the GFP sequence in the construct of BBa_K1119008 and BBa_K1119009 for characterization of MLS, which gave us the expected results.
• GFP BBa_K648013(Penn-State iGEM, 2011): green fluorescent protein reporter (BBa_E0040) with standard 25 prefix and suffix was used as a reporter for characterization of MLS and CMV promoter.

We've Also Characterized the Following Parts

• CMV promoter - GFP, BBa_K1119008: CMV promoter was assembled with GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed.
• CMV promoter - Mitochondria Leader Sequence fused with GFP , BBa_K1119009: CMV promoter was assembled with MLS and GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed and directed into mitochondria.