"
Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
(Difference between revisions)
Sabinater09 (Talk | contribs) |
Sabinater09 (Talk | contribs) |
||
| Line 45: | Line 45: | ||
<br> | <br> | ||
| - | For each sample: | + | For each sample add: |
35 ul ddH20 | 35 ul ddH20 | ||
10 ul 5X Phusion buffer | 10 ul 5X Phusion buffer | ||
| Line 52: | Line 52: | ||
1 ul appropriate diluted template DNA | 1 ul appropriate diluted template DNA | ||
0.5 ul Phusion Polymerase | 0.5 ul Phusion Polymerase | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font size="4"> '''''taq'' polymerase PCR (50ul reaction)''' </font> | ||
Revision as of 20:47, 27 September 2013
| ||
|
|
Cholera-Enzyme Protocols
Phusion PCR (50ul reaction)
For each sample add: 35 ul ddH20 10 ul 5X Phusion buffer 1.5 ul 10mM dNTP's 1 ul each Primer (50 uM stock) 1 ul appropriate diluted template DNA 0.5 ul Phusion Polymerase
taq polymerase PCR (50ul reaction) |
|
