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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
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Revision as of 21:10, 27 September 2013
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Cholera-Enzyme Protocols
Phusion PCR (50ul reaction)
For each sample add: 35 ul ddH2O 10 ul 5X Phusion buffer 1.5 ul 10mM dNTP's 1 ul each Primer (50 uM stock) 1 ul appropriate diluted template DNA 0.5 ul Phusion Polymerase
Taq polymerase PCR (50ul reaction)
For each sample add: 40 ul ddH2O 5 ul 10X TAQ buffer 1.5 ul 10 mM dNTP's 1 ul each Primer (50 uM stock) 1 ul appropriate diluted template DNA 0.5 ul TAQ Polymerase
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