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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
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Set the PCR machine to run the following cycle | Set the PCR machine to run the following cycle | ||
*98°C for 02:00 | *98°C for 02:00 | ||
| + | Repeat the next three steps 35X | ||
**98°C for 00:30 | **98°C for 00:30 | ||
**65°C for 00:30 | **65°C for 00:30 | ||
**72°C for 04:00 | **72°C for 04:00 | ||
| - | |||
*72°C for 8:00 | *72°C for 8:00 | ||
Hold at 4°C | Hold at 4°C | ||
Revision as of 21:41, 27 September 2013
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Cholera-Enzyme Protocols
Salt LB recipe
To 950 ml distilled H2O add
Adjust pH to 7.8
Fill to 1000 ml and autoclave
Phusion PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle
Repeat the next three steps 35X
Hold at 4°C
Taq polymerase PCR (50ul reaction)
For each sample add:
95 for 2 95 for .5 50 for .5 72 for 1 72 for 1
===Any deviations from these protocols are listed in the notebook when they were used=== |
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