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Team:British Columbia/Notebook/Protocols
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==Protocols== | ==Protocols== | ||
| + | ===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|PCR]]=== | ||
| + | Instruction for colony PCR and regular PCR | ||
| + | |||
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ||
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | ||
Revision as of 23:27, 27 September 2013
iGEM Home
Protocols
PCR
Instruction for colony PCR and regular PCR
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
Electrocompetent Cell Production
Gas chromatography-mass spectrometry for biosynthetic product detection
GC-MS Protocol for assaying biosynthetic product production.
Make Combinatorial Part Libraries
Make combinatorial part libraries from compatible parts
