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| | {{Template:Team:Duke}} | | {{Template:Team:Duke}} |
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| + | [[File:MainGene.jpg|700px|center]] |
| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:Duke_logo.png|900px|center]]
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| - | Project Description
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| - | Following an initial period of growth after the publication of the first synthetic
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| - | gene circuits, development in the field has stalled. This is due in part to the limited
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| - | number of well-characterized parts with desired features. For instance, the function
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| - | of the repressilator and genetic toggle switch both rely on repressible promoters
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| - | with high cooperativity – provided in these cases by multimerization of the
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| - | repressor proteins. The TALE family of transcription factors (TFs) and the CRISPR/
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| - | Cas9 system show promise in expanding the parts list to bind to near-arbitrary
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| - | target sequences, but because they bind to DNA as monomers, promoters under
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| - | their control cannot show cooperativity in their response.
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| - | <br><br>
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| - | It has been shown theoretically and in vivo that repressors binding as monomers
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| - | to multiple binding sites can introduce cooperativity in to a system. With this
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| - | in mind, we are developing an organism-independent approach that leverages
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| - | programmable TFs to create library of independent and orthogonal repressorpromoter pairs with a range of expression parameters (viz. cooperativity, basal
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| - | and maximal expression rate, response time) of potentially unlimited size. It is our
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| - | aim that this approach will enable the field to move beyond toy circuits and begin
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| - | exploring higher-order dynamics.
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| - | |[[Image:team_test.png|border|500px|center|Your team picture]]
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| - | |align="center"|[[Team:Duke | Team Duke]]
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| - | |}
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