Team:TU-Eindhoven/ProteinSelection
From 2013.igem.org
Pascalaldo (Talk | contribs) |
Pascalaldo (Talk | contribs) |
||
Line 4: | Line 4: | ||
==CEST Based Marker Proteins== | ==CEST Based Marker Proteins== | ||
{{:Team:TU-Eindhoven/Template:Lead}}To create bacteria with the ability to generate contrast on a CEST MRI scan, polypeptides that have that ability had to be found. Various solutions based on short Lysine, Arganine, Threonine or Serine rich sequences were proposed.{{:Team:TU-Eindhoven/Template:Ref | id=McMahonDIACEST | author=M.T. McMahon | title=New "Multicolor" Polypeptide Diamagnetic Chemical Exchange Saturation Transfer (DIACEST) Contrast Agents for MRI | journal=Magnetic Resonance in Medicine | edition=60 | pages=803-812 | year=2008 }} However, it is hard to predict how well these sequences will express in bacteria and whether they are sufficiently stable in vivo. To avoid these problems a new approach was taken. The suitability as a CEST Based Marker was estimated for proteins of which the structure is already clarified.{{:Team:TU-Eindhoven/Template:LeadEnd}} | {{:Team:TU-Eindhoven/Template:Lead}}To create bacteria with the ability to generate contrast on a CEST MRI scan, polypeptides that have that ability had to be found. Various solutions based on short Lysine, Arganine, Threonine or Serine rich sequences were proposed.{{:Team:TU-Eindhoven/Template:Ref | id=McMahonDIACEST | author=M.T. McMahon | title=New "Multicolor" Polypeptide Diamagnetic Chemical Exchange Saturation Transfer (DIACEST) Contrast Agents for MRI | journal=Magnetic Resonance in Medicine | edition=60 | pages=803-812 | year=2008 }} However, it is hard to predict how well these sequences will express in bacteria and whether they are sufficiently stable in vivo. To avoid these problems a new approach was taken. The suitability as a CEST Based Marker was estimated for proteins of which the structure is already clarified.{{:Team:TU-Eindhoven/Template:LeadEnd}} | ||
+ | |||
+ | ===Scanning for Candidates=== | ||
+ | To find suitable proteins the [pdb.org RCSB Protein Data Bank] was queried for all entries containing proteins. | ||
+ | {{:Team:TU-Eindhoven/Template:Code }}<nowiki> | ||
+ | <orgPdbQuery> | ||
+ | <version>head</version> | ||
+ | <queryType>org.pdb.query.simple.ChainTypeQuery</queryType> | ||
+ | <description>Chain Type: there is a Protein chain</description> | ||
+ | <containsProtein>Y</containsProtein> | ||
+ | <containsDna>?</containsDna> | ||
+ | <containsRna>?</containsRna> | ||
+ | <containsHybrid>?</containsHybrid> | ||
+ | </orgPdbQuery> | ||
+ | </nowiki>{{:Team:TU-Eindhoven/Template:CodeEnd }} | ||
+ | |||
+ | |||
{{:Team:TU-Eindhoven/Template:RefAgain | id=McMahonDIACEST }} | {{:Team:TU-Eindhoven/Template:RefAgain | id=McMahonDIACEST }} | ||
Revision as of 12:00, 2 July 2013
![](https://static.igem.org/mediawiki/2013/6/6d/TU-Eindhoven_Images_LogoTransp.png)
![](https://static.igem.org/mediawiki/2013/3/32/TU-Eindhoven_Images_MRiGEM.png)
![](http://2013.igem.org/wiki/images/4/45/TU-Eindhoven_iGEM_small.png)
CEST Based Marker Proteins
To create bacteria with the ability to generate contrast on a CEST MRI scan, polypeptides that have that ability had to be found. Various solutions based on short Lysine, Arganine, Threonine or Serine rich sequences were proposed.McMahonDIACESTM.T. McMahon, New "Multicolor" Polypeptide Diamagnetic Chemical Exchange Saturation Transfer (DIACEST) Contrast Agents for MRI. Magnetic Resonance in Medicine 60, 803-812 (2008) However, it is hard to predict how well these sequences will express in bacteria and whether they are sufficiently stable in vivo. To avoid these problems a new approach was taken. The suitability as a CEST Based Marker was estimated for proteins of which the structure is already clarified.
Scanning for Candidates
To find suitable proteins the [pdb.org RCSB Protein Data Bank] was queried for all entries containing proteins.
<orgPdbQuery> <version>head</version> <queryType>org.pdb.query.simple.ChainTypeQuery</queryType> <description>Chain Type: there is a Protein chain</description> <containsProtein>Y</containsProtein> <containsDna>?</containsDna> <containsRna>?</containsRna> <containsHybrid>?</containsHybrid> </orgPdbQuery>
McMahonDIACEST
References