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Team:INSA Toulouse/contenu/lab practice/notebook/calendar/recomb
From 2013.igem.org
(Difference between revisions)
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Assembling FimE recombinase with rbs by 3A assembling into pSB1C3<br> | Assembling FimE recombinase with rbs by 3A assembling into pSB1C3<br> | ||
Mutagenese of PhiC31 from the Suiti Parts (A24) to delete the EcoRI restriction site<br> | Mutagenese of PhiC31 from the Suiti Parts (A24) to delete the EcoRI restriction site<br> | ||
| - | PCR to extract Tp901.1 from the Bonnet parts | + | PCR to extract Tp901.1 from the Bonnet parts<br><br> |
| - | + | ||
16/07<br> | 16/07<br> | ||
Purification of the PCR and verification by gel.<br> | Purification of the PCR and verification by gel.<br> | ||
| Line 178: | Line 177: | ||
Assembling PhiC31 mutated with the terminator into pSB1C3.<br><br> | Assembling PhiC31 mutated with the terminator into pSB1C3.<br><br> | ||
19/07<br> | 19/07<br> | ||
| - | |||
Assembling of rbs.FimE with terminator into pSB1C3.<br> | Assembling of rbs.FimE with terminator into pSB1C3.<br> | ||
Arrival of the synthesis.<br><br> | Arrival of the synthesis.<br><br> | ||
20/07<br> | 20/07<br> | ||
| - | Verification of the clonage | + | Verification of the clonage.<br><br> |
| - | + | ||
</li> | </li> | ||
<li><span class="spantitle2">Week 7 (22-28 July)</span><br> | <li><span class="spantitle2">Week 7 (22-28 July)</span><br> | ||
22/07<br> | 22/07<br> | ||
| - | Assembling | + | Assembling PhiC31*... |
</li> | </li> | ||
</ul></li> | </ul></li> | ||
Revision as of 07:10, 29 September 2013
Calendar
Recombinases
July 2013
- Week 6 (15-21 July)
15/07
Assembling Bxb1 with terminator by 3A assembling into pSB1A3
Assembling FimE recombinase with rbs by 3A assembling into pSB1C3
Mutagenese of PhiC31 from the Suiti Parts (A24) to delete the EcoRI restriction site
PCR to extract Tp901.1 from the Bonnet parts
16/07
Purification of the PCR and verification by gel.
Miniprep for the verification of the clonage, Bxb1.term and rbs.FimE.
For the mutagenese of PhiC31, restriction by DpnI and transformation.
17/07
Assembling Tp901.1 with terminator by 3A assembling.
PCR of extraction of the vector A24 with Phic31 mutated.
18/07
Assembling PhiC31 mutated with the terminator into pSB1C3.
19/07
Assembling of rbs.FimE with terminator into pSB1C3.
Arrival of the synthesis.
20/07
Verification of the clonage.
- Week 7 (22-28 July)
22/07
Assembling PhiC31*...
August 2013
- Week ...
- Week ...
