Team:Paris Saclay/Notebook/July/18

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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
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===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
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===='''1 - PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI'''====
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===='''1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI'''====
Abdou, Anaïs
Abdou, Anaïs
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/July/19|<big>next day</big>]]
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|[[Team:Paris Saclay/Notebook/July/19|<big>Next day</big>]]
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{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 09:47, 30 September 2013

Contents

Notebook : July 18

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI

Abdou, Anaïs

Used quantities :

  • Buffer Dream Taq : 5µL
  • dNTP : 1µL
  • Oligo VF : 2µL
  • Oligo VR : 2µL
  • H2O : 37.75µL
  • DNA : 2µL
  • Dream Taq : 0.25µL

PCR program :

PsPCR1807.jpg


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