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Team:Paris Saclay/Notebook/July/18
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - PCR of | + | ===='''1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI'''==== |
Abdou, Anaïs | Abdou, Anaïs | ||
Latest revision as of 09:47, 30 September 2013
Contents |
Notebook : July 18
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI
Abdou, Anaïs
Used quantities :
- Buffer Dream Taq : 5µL
- dNTP : 1µL
- Oligo VF : 2µL
- Oligo VR : 2µL
- H2O : 37.75µL
- DNA : 2µL
- Dream Taq : 0.25µL
PCR program :
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