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Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

Glycerol 85% : 425
Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.

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@@ Line 1: / Line 1: @@
 {{Team:Paris_Saclay/incl_debut_generique}}
 ='''Notebook : July 12'''=
-==''summary''==
-For fnr regulator system:
-*Performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
-*Design of oligo for amplifying AmiCP+RBS.
-For PCBs sensor system:
-*Had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
-*Plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
 =='''Lab work'''==
-A.aero/anaerobic regulation system:
-*BioBrick RBS+LacZ+terminator in plasmid PSB1C3
+==='''A - Aerobic/Anaerobic regulation system'''===
-<br>
+===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
+===='''1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017'''====
+Zhou
+{|
+| style="border:1px solid black;padding:5px;background-color:#DE;" |
+Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.
+|}
+Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
+===='''2 -Design of RBS-AmilCP oligos'''====
+Abdou, Anaïs
+We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
+==='''B - PCB sensor system'''===
+===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''====
+===='''1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2'''====
+Zhou
+Used quantities :
+* Glycerol 85% : 425
+* Culture : 1µL
+===='''2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α'''====
+Abdou, Sheng
+Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
+===='''3 - Streak of BBa_K1155002 and BphR2'''====
+Anaïs
+We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
-<br>
 {| border="1" align="center"
 |[[Team:Paris Saclay/Notebook/July/11|<big>Previous day</big>]]
@@ Line 22: / Line 57: @@
 |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
-|<big>Next week</big>
+|[[Team:Paris Saclay/Notebook/July/15|<big>Next week</big>]]
 |}
 {{Team:Paris_Saclay/incl_fin}}

Team:Paris Saclay/Notebook/July/12

From 2013.igem.org