Team:Paris Saclay/Notebook/July/12

From 2013.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 7: Line 7:
==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
-
===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
+
===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
-
===='''1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010'''====
+
===='''1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017'''====
Zhou
Zhou
Line 15: Line 15:
{|
{|
| style="border:1px solid black;padding:5px;background-color:#DE;" |
| style="border:1px solid black;padding:5px;background-color:#DE;" |
-
Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. We obtain two colonies of 07/10/13 transformation. We will extract Bba_B0010 and do the transformation again.
+
Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.  
|}
|}
-
Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
+
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
===='''2 -Design of RBS-AmilCP oligos'''====
===='''2 -Design of RBS-AmilCP oligos'''====
Line 25: Line 25:
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
-
 
-
===='''3 -Extraction of Bba_B0010 from DH5α'''====
 
-
 
-
Sheng
 
-
 
-
Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
 
==='''B - PCB sensor system'''===
==='''B - PCB sensor system'''===
-
===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''====
+
===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''====
-
===='''1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2'''====
+
===='''1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2'''====
Zhou
Zhou
Line 45: Line 39:
* Culture : 1µL
* Culture : 1µL
-
===='''2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α'''====
+
===='''2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α'''====
Abdou, Sheng
Abdou, Sheng
Line 51: Line 45:
Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
-
===='''3 - Streak of BphA1 and BphR2'''====
+
===='''3 - Streak of BBa_K1155002 and BphR2'''====
Anaïs
Anaïs
-
We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.  
+
We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.  
 +
 
{| border="1" align="center"
{| border="1" align="center"

Latest revision as of 09:55, 30 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

  • Glycerol 85% : 425
  • Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.


Previous day Back to calendar Next week

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Notebook/July/12"